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You can delete the applied validation rule using the Delete method of the IValidation interface.
Refer to the following example code to know how you can delete validation rule applied to a cell or a range of cells in a worksheet.
//Add validation
worksheet.Range["A1:A3"].Validation.Add(ValidationType.Whole, ValidationAlertStyle.Stop, ValidationOperator.Between, 1, 8);
worksheet.Range["B1:B3"].Validation.Add(ValidationType.Whole, ValidationAlertStyle.Stop, ValidationOperator.Between, 11, 18);
//Delete validation.
worksheet.Range["A1:A2"].Validation.Delete();